Active teams in bold.

• Brantford Redskins (member 1952–1953)
• Camp Borden Flyers (member 1925–1940)
• Dundas Rugby Club (member 1910–1911)
• Hamilton Alerts (member 1911–1940)
• Hamilton Panthers (member 1926–1937)
• Hamilton Rowing Club (member 1913–1925)
• Hamilton Tiger-Cats (member 1883–1949)
• Hamilton West End Pleasure Club (member 1903)
• Hamilton Wildcats (member 1941–1947)
• Kingston Limestones (member 1899–1907)
• Kitchener-Waterloo Dutchmen (member 1928–1959)
• London Lords (member 1904–1960)
• McMaster University (member 1951)
• Montreal Nationals (member 1938)
• Osgoode Hall Rugby Club (member 1891–1898)
• Ottawa RedBlacks (member 1898–1902)
• Ottawa Trojans (member 1943–1947)
• Ottawa College Rugby Club (member 1883–1893)
• Peterborough ORFUns (member 1904–1939)
• Port Huron Raiders (member 1958–1960)
• Queen's Golden Gaels (member 1883–1897)
• Rochester Rockets (member 1956)
• Sarnia Golden Bears (member 1928–1960)
• St. Brigid's Football Club (member 1921–1922)
• St. Michael's College (member 1910–1934)
• St. Patrick's Saints (member 1914)
• Toronto Argonauts (member 1883–1906)
• Toronto Beaches (member 1924–1957)
• Toronto Capitals (member 1919)
• Toronto HMCS York Navy (member 1942–1943)
• Toronto Indians (member 1941–1947)
• Toronto Parkdale Canoe Club (member 1909–1923)
• Toronto RCAF Hurricanes (member 1942–1943)
• Toronto Victorias (member 1904–1907)
• Torontos (member 1908–1921)
• University of Toronto Varsity Blues (member 1890–1897)
• University of Toronto Varsity Seconds (member 1922–1931)
• Westmount Football Club (member 1907–1939)
• Windsor Royals (member 1929–1952)